Product Name :
Phospho-Smad5 (S463 + S465) Recombinant Rabbit Monoclonal Antibody [SY09-03]
Predicted band size :
52 kDa
Observed band size :
52 kDa
Synonyms:
DKFZp781C1895 antibody DKFZp781O1323 antibody Dwfc antibody hSmad5 antibody JV5 1 antibody JV5-1 antibody MAD homolog 5 antibody MAD, mothers against decapentaplegic homolog 5 antibody MADH 5 antibody MADH5 antibody 展开
Function :
Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling.2377610-54-1 supplier Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation.1222174-93-7 manufacturer Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic phospho-peptide corresponding to residues surrounding Ser463 and 465 of Human Smad5. The immunogen may cross with p-smad1/9.
Species reactivity:
Human, Mouse, Rat
Verify application:
WB, IF-Cell, IHC-P
Coupling :
unconjugated
Clone number:
SY09-03
RRID:
AB_3069717
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.PMID:33750623
Storage buffer solution:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 52 kDa
Positive control :
HeLa treated with 20 ng/mL TGFβ1 for 15 minutes whole cell lysate, Rat brain tissue lysate, mouse brain tissue lysate, HeLa cells treated with 20ng/mL TGFβ1 for 15 minutes, SKOV-3, HepG2, human tonsil tissue, human breast carcinoma tissue, human liver tissue, mouse brain tissue.